Recombinant DNA Technology: PCR, Restriction analysis, DNA ligation, Protein Expression, and Western blotting

Recombinant DNA Technology: PCR, Restriction analysis, DNA ligation, Protein Expression, and Western blotting

Last Date of Registration :  15th June, 2026

Mode of the LAB Workshop: IN – PERSON at IISER Bhopal

 

Kindly fill the registration form attached below. Payment link will be sent to the eligible candidates.

SKU: IISER Bhopal | Date : 29th June - 4th July, 2026 Category:

Profile of the Instructor

Dr. Ram Kumar Mishra: Joined IISER Bhopal in November 2011, after  completing his PhD and postdoctoral research from TIFR Mumbai and  NIH Bethesda, respectively. He has been teaching theory (Cell Biology,  Developmental Biology, Cell Signaling, and Animal Physiology) and  laboratory courses (Molecular biology, Protein biochemistry, and  Animal Physiology) to UG and PG students since then.

Dr. Chandan Sahi: Dr. Chandan joined IISER Bhopal in July 2011, after  completing his PhD and postdoctoral research in Plant Molecular  Biology at the University of Delhi and the University of Wisconsin,  Madison, respectively. He has been teaching theory (Recombinant  DNA technology, Genetics, Stress Biology, and Plant Physiology) and  laboratory courses (Molecular biology, Protein biochemistry, and Plant  Physiology) to UG and PG students since then.

List of Experiments conducted in the LAB Workshop

Name Description
Bacterial
Transformation		 Culturing E. coli and processing for plasmid transformation (CaCl2 method)
Plasmid isolation Alkaline lysis method to harvest the plasmid from E. coli
PCR amplification Setting up a PCR reaction and visualize PCR product by Agarose gel electrophoresis
Restriction
digestion		 Digestion of Plasmid DNA with restriction enzyme and analysis of the digested pattern by agarose gel electrophoresis
DNA ligation and cloning Joining two pieces of DNA with similar overhangs (cohesive ends)
Protein expression Lac operon-dependent induction of protein expression in E. coli
Protein Affinity
Purification		 Purification of recombinant protein expressed in E. coli using affinity chromatography (Glutathione beads)
Western blotting Detection of purified protein using anti-GST antibody

Eligibility & Qualification Criteria

Eligibility Criteria: 

Should have knowledge of Molecular Biology and Biotechnology

Qualification Criteria:

B.Sc (3rd year) and M.Sc

Fee for the LAB Workshop

Registration fee : Rs. 1000 

A refund of Rs. 800 will be provided to eligible candidates upon completion of the LAB Workshop.

Please note: No refunds will be issued after the registration deadline on 15th June, 2026

LAB Workshop Session Details

Date of the Workshop : 29th June - 4th July, 2026

Mode of the Workshop : IN – PERSON at IISER Bhopal

 

  • Start date: 29th June, 2026
  • End date: 3rd July, 2026
  • Exam date: 4th July, 2026

       

Since the lab workshop will be residential:

Cost/day (room+food) : Rs. 500/day (includes room and bedding  rent, and three meals)

Instructions for the lab workshop: (Things to be brought, safety rules, etc) :

A lab record book and a lab coat, other details will be sent through email to the registered candidates 

Day-wise planner

Day Details of planned activity
1 Prepare competent cells, transformation, and alkaline lysis-mediated plasmid isolation
2 Digestion of Plasmid DNA with restriction enzyme, setting up a PCR reaction, and analysis of DNA on agarose gel electrophoresis
3 DNA ligation (joining of DNA fragments) and analysis by agarose gel electrophoresis
4 Protein expression and Glutathione affinity purification
5 Bradford reagent-based protein estimation, SDS-PAGE, and Western blotting
6 Exams

Mark Split for the workshop

Internal: 40/100 (Attendance in theory and lab sessions, record book, etc)

Final exam: 60/100 (includes a written quiz, spotting, and performing experiments in the  exam)

Contact Details for Enquiry

Contact details of person at host institute for students to ask queries regarding the lab workshop:

Name: Ram Kumar Mishra  

Designation: Associate Professor  

Email ID: rkmishra@iiserb.ac.in  

Phone number: 0755-2691407

Contact details for Registration queries:

Email ID: nptelplus-workshop@nptel.iitm.ac.in

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