From: ₹750.00
Dr. Vishal Trivedi did his Ph.D. from Central Drug Research Institute, Lucknow in the field of Structural Biology. He has extensive experience to run online courses on MOOCS platform and his teaching domain lies in the area of molecular cloning, protein production and biochemical characterization. He received several awards such as young scientist award from National academy of sciences-India, young scientist award from DST-SERB, young scientist award from DAE-BRNS.
| Module name | Concepts covered | Recorded videos - number of hours
Live sessions - No. of hours |
Assessment | Learning outcomes | |
| Day 1 | Isolation of gene of interest. | 1. Lecture 1: Different approaches to identify and isolate gene of interest.
2. Lecture 2: DNA Libraries 3. Lecture 3: Polymerase chain reaction |
Live session: 3 Hrs
Recorded Video: 2 |
Attendance: 50%
Assignment 50% |
1. Isolation of genomic and m-RNA from Host cells. 2. Designing genomic or cDNA library. 3. Screening of DNA libraries 4. Primer designing5. Polymerase chain reaction |
| Day 2 | Cloning and Over-expression in Host | 1. Lecture 1: Tools for molecular cloning 2. Lecture 2: Selection of Host/Vector for protein production 3. Lecture 3: Over-expression and analysis |
Live session: 3 Hrs
Recorded Video: 2 |
Attendance: 50% Assignment 50% |
1. Selection of Host-vector for protein production
2. Protein production and analysis. |
Intended audience:
Eligibility requirement :
Graduate students with basic biology information.
Weightage for Attendance and Assignment:
| Day | Attendance | Assignment |
| Day 1 | 50% | 50% |
| Day 2 | 50% | 50% |
Certification will be provided based on following criteria:
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